THE POTENTIAL FOR APPLICATION OF ENZYME CELLULASE IN THE BAKING INDUSTRY

Authors

  • Anastasiia Kulakivska Lviv Polytechnic National University image/svg+xml Author
  • Roksolana Konechna Lviv Polytechnic National University image/svg+xml Author
  • Olena Vichko Ternopil Ivan Pului National Technical University image/svg+xml Author
  • Iryna Nazarko Ternopil Ivan Pului National Technical University image/svg+xml Author
  • Svyatoslav Polovkovych Lviv Polytechnic National University image/svg+xml Author

DOI:

https://doi.org/10.31548/humanhealth.3.2026.82

Keywords:

cellulase enzyme, bakery products, cellulose, bran, frozen semi-finished bakery products

Abstract

The shift in Ukrainian consumer preferences from traditional rye or wheat loaves toward health-promoting, wellness-oriented bakery products has enriched the food market. Industrial manufacturers have begun to actively expand their product range and introduce new recipes, particularly for non-traditional types of bread – gluten-free, sourdough and yeast-free varieties. One of the priority areas is the replacement of synthetic additives with natural ingredients, particularly enzyme-based additives. The aim of this study is to systematise the relevant literature regarding the prospects for the use of the cellulase enzyme to significantly improve the quality of bakery products.

In the course of this study, information on the prospects and advantages of cellulolytic hydrolysis as a targeted biotechnological tool for modifying grain cell wall components was summarised and organised. The key finding of the study is the justification of the high efficiency of cellulolytic hydrolysis: it was established that by cleaving β -1,4-glycosidic bonds in cellulose, the cellulase enzyme releases pentosans and bound water, thereby altering the rheology of the product. This modification positively affects the crumb structure of bread and other bakery products, increases loaf volume. Furthermore, the enzymatic treatment of wheat and oat bran added to the dough reduces its water absorption capacity, enhances its elasticity, and accelerates the baking process. The application of cellulase in combination with other hydrolases enables the achievement of optimal porosity and improves the organoleptic quality attributes of the bread. It has been demonstrated that the application of cellulase in the production of frozen semi-finished products inhibits the migration of water molecules within the dough, thereby mitigating the negative impact of ice crystals on the yeast and the fermentative activity of yeast cells after thawing.

The practical value of this study lies in providing recommendations on the use of cellulase enzyme in bread production technology to improve its organoleptic quality characteristics. The obtained findings can be applied by food industry manufacturers to develop new formulations for functional bakery products with enhanced characteristics and an extended shelf life. Implementing such biotechnological innovations will facilitate the expansion of the range of "clean label" functional products. This will ensure the high competitiveness of the domestic baking industry and fully satisfy the current demands of modern consumer.

Received 12.03.2026, accepted  28.07.2026, published 22.06.2026

References

Askari, H., Soleimanian-Zad, S., Kadivar, M., Shahbazi, S. (2025). Enhancement of wheat bread quality using xylanase cellulase from gamma radiated Trichoderma afroharzianum mutant. Scientific Reports, 15(1), 43001. https://doi.org/10.1038/s41598-025-27026-5

Chauhan, J., Shukla, R., Bishoyi, A. K., Goyal, S., Sanghvi, G. (2023). Investigation of physical, nutritional and sensory properties of wheat bread treated with purified thermostable cellulase and alpha amylase. Cogent Food & Agriculture, 9(1). https://doi.org/10.1080/23311932.2023.2261839

Chowdhury, M. H., Sarkar, F., Reem, C. S. A., Rachman, S. M., Mahamud, A. G. M. S. U., Rachman, M. A., Ashrafudoulla. M. (2024). Enzyme applications in baking: From dough development to shelf-life extension. International Journal of Biological Macromolecules, 282(Pt 4), 137020. https://doi.org/10.1016/j.ijbiomac.2024.137020

Commission regulation (EU) a common authorisation procedure for food additives, food enzymes and food flavourings (2011, March). Retrieved from https://eur-lex.europa.eu/eli/reg_impl/2011/234/oj

Dahiya, S., Bajaj, B. K., Kumar, A., Tiwari, S. K., Singh, B. (2020). A review on biotechnological potential of multifarious enzymes in bread making. Process Biochemistry, 99, 290–306. https://doi.org/10.1016/j.procbio.2020.09.002

De Souza, T. S. P., & Kawaguti, H. Y. (2021). Cellulases, hemicellulases, and pectinases: Applications in the food and beverage industry. Food and Bioprocess Technology, 14(8), 1446–1477. https://doi.org/10.1007/s11947-021-02678-z

Ejaz, U., Sohail, M., Ghanemi, A. (2021). Cellulases: From Bioactivity to a Variety of Industrial Applications. Biomimethics, 6(3), 44. https://doi.org/10.3390/biomimetics6030044

Enzym Group. (n.d.). Retrived from https://enzymgroup.com/uk/solutions/bakery-solutions.

Fu, Z., Sun, X., Zhu, K., Guo, X.-N. (2025). Improving the quality of frozen steamed stuffed buns after freeze-thaw cycles: Synergistic effects of cellulase, xylanase and sourdough. Food Bioscience, 68, 106524. https://doi.org/10.1016/j.fbio.2025.106524

Jonson, J. A., Miller, B. S. (1948) High levels of alfha-amylase in baking. L evaluation of the effect of alpha-amylase from various sources. Cereal chemistry, 25, 168-178.

Kocabaş, D. S., & Grumet, R. (2019). Evolving regulatory policies regarding food enzymes produced by recombinant microorganisms. GM Crops & Food, 10(4), 191–207. https://doi.org/10.1080/21645698.2019.1649531

Kumar, A., Dhiman, S., Krishan, B., Samtiya, M., Kumari, A., Pathak, N., Kumari., A., Aluko, R. E., Dhewa., T. (2024). Microbial enzymes and major applications in the food industry: a concise review. Food Production Processing and Nutrition, 6(1). https://doi.org/10.1186/s43014-024-00261-5

Law of Ukraine No. 35 “On the State Biosafety System for the Creation, Testing, Transportation and Use of Genetically Modified Organisms”. (2007, May). Retrieved from https://zakon.rada.gov.ua/laws/show/1103-16/ed20201016#Text

Lewko, P., Wójtowicz, A., & Kamiński, D. M. (2024). The Influence of Processing Using Conventional and Hybrid Methods on the Composition, Polysaccharide Profiles and Selected Properties of Wheat Flour Enriched with Baking Enzymes. Foods, 13(18), 2957. https://doi.org/10.3390/foods13182957

Liang, X., Hong, J., Liu, B., Cui, Y., Zhang, S., Choi. L., Guan, E., Omer, S. H. S., Hu, Z., Zheng, X. (2024). Effect of cellulase and pentosanase with lactic acid bacteria to increase gluten formation and elasticity in whole-wheat dough sheets. LWT, 205, 116521. https://doi.org/10.1016/j.lwt.2024.116521

Liu, W., Brennan, M., Brennan, C., You, L. (2023). Individual and combined effects of α-amylase, xylanase, and cellulase on the breadmaking and nutritional properties of steamed bun enriched in wheat bran. Journal of Food Science, 88(8), 3228-3238 https://doi.org/10.1111/1750-3841.16665

Liu, W., Brennan, M., Tu, D., & Brennan, C. (2023). Influence of α-amylase, xylanase and cellulase on the rheological properties of bread dough enriched with oat bran. Scientific Reports, 13(1). https://doi.org/10.1038/s41598-023-31591-y

Liu, W., Brennan, M., Tu, D., Brennan, C., Huang, W. (2023). Effect of enzyme compositions on the rheological properties of bread dough enriched in buckwheat flour. Food Science and Technology, 43. https://doi.org/10.5327/fst.114322

Liu, X., Zhu, X., Zhu, Y., Wang, C., Li, H., Chen, S. (2024). Effect of cellulase on dough structure and quality characteristics of tough biscuits enriched with potato whole flour. Journal of Food Science, 89(6), 3484–3493. https://doi.org/10.1111/1750-3841.17091

Liu, W., Brennan, M. A., Serventi, L., Brennan, C. S. (2017). Effect of cellulase, xylanase and α-amylase combinations on the rheological properties of Chinese steamed bread dough enriched in wheat bran. Food Chemistry, 234, 93–102. https://doi.org/10.1016/j.foodchem.2017.04.160

Market.us. (2025). Baking Enzymes Market Size, Share CAGR of 5.5%. Retrieved from https://market.us/report/baking-enzymes-market/

Maat, J., Roza, M. (1989, March 23). US5108765A - Composition for improving the properties of dough and method of using same - Google Patents. https://patents.google.com/patent/US5108765A/en

Nguyen, N. D. T., Phan, T. T. H., Tran, T. T. T., Ton, N. M. M., Vo., D. L. T, Le. V. V. M. (2022). Enzymatic treatment of spent green tea leaves and their use in High-Fiber cookie production. Food Technology and Biotechnology, 60(3), 396–405. https://doi.org/10.17113/ftb.60.03.22.7474

Nguyen, S. N., Vien, M. D., Le, T. T. T., Tran, T. T. T., Ton, N. M. N., Le, V. V. M. (2021). Effects of enzymatic treatment conditions on dietary fibre content of wheat bran and use of cellulase‐treated bran in cookie. International Journal of Food Science & Technology, 56(8), 4017–4025. https://doi.org/10.1111/ijfs.15022

Oliynyk, S. G., Zaparenko, G. V., & Dyakov, O. G. (2016). Optimization of the composition of enzyme preparations for improving the quality of grain bread. Food Science and Technology, 10(1). https://doi.org/10.21691/fst.v10i1.76

Ozatay, S. (2020). Recent applications of enzymes in food industry. Journal of Current Researches on Engineering Science and Technology, 6(6 (1)), 17–30. https://doi.org/10.26579/jocrest.52

Putseys, J. A., & Schooneveld‐Bergmans, M. E. (2019). Enzymes used in baking. In Industrial Enzyme Applications (pp. 95–123). https://doi.org/10.1002/9783527813780.ch2_1

Register of food enzymes to be considered for inclusion in the union list (2024, July). Retrieved from https://food.ec.europa.eu/document/download/910b596e-aa10-4c5f-9831-677c28f4a973_en?filename=fs_food-improvement-agents_enzymes_register.pdf

Sharma, A., Tewari, R., Rana, S. S., Soni, R., Soni, S. K. (2016). Cellulases: Classification, methods of determination and industrial applications. Applied Biochemistry and Biotechnology, 179(8), 1346–1380. https://doi.org/10.1007/s12010-016-2070-3

Silano, V., Baviera, J. M. B., Bolognesi, C. et al. (2019). Characterisation of microorganisms used for the production of food enzymes. EFSA Journal, 17(6).https://doi.org/10.2903/j.efsa.2019.5741

Vakulenko, O. O., & Zhuravel, O. S. The increasing of the energy efficiency for production of bakery products // Abstracts of the VII International Scientific and Technical Conference “Lighting and Electrical Engineering: History, Problems, Prospects” (May 15-17, 2024). – Ternopil – P. 93-94.

Wang, X., Pei, D., Teng, Y., Liang, J. (2017). Effects of enzymes to improve sensory quality of frozen dough bread and analysis on its mechanism. Journal of Food Science and Technology, 55(1), 389–398. https://doi.org/10.1007/s13197-017-2950-8

Yoon, J., Lee, K., & Lee, H. (2022). Physicochemical properties of enzyme treated-dietary fibers from whole grain and the quality characteristics of cakes. Korean Journal of Food Science and Technology, 54(5), 490–497. https://doi.org/10.9721/kjfst.2022.54.5.490

Published

2026-09-22

Issue

Section

Food technologies

How to Cite

Kulakivska, A., Konechna, R., Vichko, O., Nazarko, I., & Polovkovych, S. (2026). THE POTENTIAL FOR APPLICATION OF ENZYME CELLULASE IN THE BAKING INDUSTRY. Human and Nation’s Health, 4(3), 82-95. https://doi.org/10.31548/humanhealth.3.2026.82