DEVELOPMENT OF FUNCTIONAL BEVERAGES AND DIETARY SUPPLEMENTS BASED ON TABLE BEET AS A NATURAL SOURCE OF PREBIOTICS
DOI:
https://doi.org/10.31548/humanhealth.4.2026.98Keywords:
dietary supplements, gut microbiome, betalains, fractionated juice, nitrates, One HealthAbstract
Abstract. This work focuses on developing technological solutions to create functional beverages and dietary supplements with pronounced prebiotic properties, based on table beet (Beta vulgaris rubra). It presents findings from research on innovative dietary supplements derived from beet juice via molecular fractionation and ultrafiltration. The biological activity of the resulting concentrate – enriched with natural nitrates, glutamic acid, and betalain pigments – was analyzed. This research contributes to the understanding of global trends in the production of fermented functional beverages and dietary supplements, providing a scientific basis for developing comprehensive technological solutions for innovative products with significant prebiotic properties. The study investigates low-temperature fractionation of the raw material using ultrafiltration, a method that maximizes the preservation of native betalains, inorganic nitrates, and oligosaccharides without requiring harsh thermal pasteurization. To ensure the microbiological safety of the final liquid products, nanotechnological approaches – specifically the modification of materials with biocidal metal nanoparticles – have been integrated at critical control points within the production process. Synthesizing scientific, theoretical, and biomedical aspects of the role of microorganisms and natural plant-based antioxidants, this study integrates nanobiotechnological approaches to ensure the microbiological safety and purity of finished products. It further demonstrates the efficacy of beetroot concentrate in optimizing the microbiome, enhancing cardiorespiratory endurance, and supporting systemic disease prevention within the framework of the "One Health" concept. Integrating the authors' previous work on phytosupplements and bioactive phytoextracts in food biotechnology-combined with research from Latvian colleagues and current international studies on the ultrafiltration of beetroot-enhances the proposed concept by introducing effective methods for isolating and developing dietary supplements derived from clean, sustainable, organic prebiotic raw materials. The resulting prebiotic concentrate has been shown to effectively stimulate the growth of beneficial gut microbiota; its functional value and the environmental safety of its production process have also been confirmed. The implementation of the "One Health" concept in Ukraine depends directly on the ability to ensure the safety of food supply chains at the intersection of agricultural technology, nutrition, and medicine.
References
Abedimanesh, N., Asghari, S., Mohammadnejad, K., Daneshvar, Z., Rahmani, S., Shokoohi, S., Farzaneh, A. H., Hosseini, S. H., Jafari Anarkooli, I., Noubarani, M., Andalib, S., Eskandari, M. R., & Motlagh, B. (2021). The anti-diabetic effects of betanin in streptozotocin-induced diabetic rats through modulating AMPK/SIRT1/NF-κB signaling pathway. Nutrition & metabolism, 18(1), 92. https://doi.org/10.1186/s12986-021-00621-9
Adeyemi, J. O., & Fawole, O. A. (2023). Metal-based nanoparticles in food packaging and coating technologies: A review. Biomolecules, 13(7), 1092. https://doi.org/10.3390/biom13071092
Alarkwazi, R. K., & Al-Tabatabai, H. H. M. (2026). Estimation of some vitamins in beetroot juice and their relationship to cancer. Indian Journal of Agricultural Research, 60(3), 474–480. https://doi.org/10.18805/IJARe.AF-998
Al-Harbi, L. N., Alshammari, G. M., Al-Dossari, A. M., Subash-Babu, P., Binobead, M. A., Al Hussain, M. H., AlSedairy, S. A., Al-Nouri, D. M., & Shamlan, G. J. B. (2021). Beta vulgaris L. (beetroot) methanolic extract prevents hepatic steatosis and liver damage in T2DM rats by hypoglycemic, insulin-sensitizing, antioxidant effects, and upregulation of PPARα. Biology, 10 (12), Article 1306. https://doi.org/10.3390/biology10121306
Al-Harbi, L. N., Pandurangan, S. B., Al-Dossari, A. M., Shamlan, G., Salamatullah, A. M., Alshatwi, A. A., & Alotiby, A. A. (2021). Beta vulgaris rubra L. (Beetroot) Peel Methanol Extract Reduces Oxidative Stress and Stimulates Cell Proliferation via Increasing VEGF Expression in H2O2 Induced Oxidative Stressed Human Umbilical Vein Endothelial Cells. Genes, 12(9), 1380. https://doi.org/10.3390/genes12091380
Aliahmadi, M., Amiri, F., Bahrami, L. S., Hosseini, A. F., Abiri, B., & Vafa, M. (2021). Effects of raw red beetroot consumption on metabolic markers and cognitive function in type 2 diabetes patients. Journal of Diabetes and Metabolic Disorders, 20(1), 673–682. https://doi.org/10.1007/s40200-021-00798-z
Apaydın, A. S., & Onsekizoğlu Bağcı, P. (2025). Applications of membrane separation techniques in fruit juice processing. The Journal of Food, 50(3), 442-465. https://dergipark.org.tr/en/pub/gida/article/1635105
Babarykin, D. (2022). On new trends in increasing the effectiveness of food supplements. Latvian Academy of Sciences Yearbook, 71–75. https://www.lza.lv/images/Annual_reports/YearBook_2022.pdf
Babarykin, D., Smirnova, G., Krumina, G., Vasiljeva, S., Krumina, Z., Basova, N., & Fedotova, A. (2018). Stimulating effect of red beetroot (Beta vulgaris) juice, fractioned by membrane ultrafiltration, on iron absorption in chicken intestines. Journal of Biosciences and Medicines, 6(11), 37–49. https://doi.org/10.4236/jbm.2018.611004
Babarykin, D., Smirnova, G., Markovs, J., Vasiljeva, S., Basova, N., Simanis, R., & Viksna, L. (2019). Therapeutic effect of fractionated by ultrafiltration red beetroot (Beta vulgaris L.) juice in rats with food-induced fatty liver. European Journal of Biological Research, 9(1), 1–9. https://doi.org/10.5281/zenodo.2541075
Babarykin, D., Smirnova, G., Pundinsh, I., Vasiljeva, S., Krumina, G., & Agejchenko, V. (2019). Red beet (Beta vulgaris) impact on human health. Journal of Biosciences and Medicines, 7(3), 61–79. https://doi.org/10.4236/jbm.2019.73007
Bianchi, F., Pünsch, M., & Venir, E. (2021). Effect of Processing and Storage on the Quality of Beetroot and Apple Mixed Juice. Foods (Basel, Switzerland), 10(5), 1052. https://doi.org/10.3390/foods10051052
Bochnak-Niedźwiecka, J., Szymanowska, U., & Świeca, M. (2020). Studies on the development of vegetable-based powdered beverages: Effect of the composition and dispersing temperature on potential bioaccessibility of main low-molecular antioxidants and antioxidant properties. LWT - Food Science and Technology, 134, 110222. https://doi.org/10.1016/j.lwt.2020.110222
Ceclu, L., & Nistor, O. (2020). Red beetroot: Composition and health effects - A review. Journal of Nutritional Medicine and Diet Care, 5(2). https://doi.org/10.23937/2572-3278/1510043
Chhikara, N., Kushwaha, K., Sharma, P., Gat, Y., & Panghal, A. (2019). Bioactive compounds of beetroot and utilization in food processing industry: A critical review. Food chemistry, 272, 192–200. https://doi.org/10.1016/j.foodchem.2018.08.022
Clifford, T., Howatson, G., West, D. J., & Stevenson, E. J. (2015). The potential benefits of red beetroot supplementation in health and disease. Nutrients, 7(4), 2801–2822. https://doi.org/10.3390/nu7042801
Cui, R., Fei, Y., & Zhu, F. (2022). Physicochemical, structural and nutritional properties of steamed bread fortified with red beetroot powder and their changes during breadmaking process. Food chemistry, 383, 132547. https://doi.org/10.1016/j.foodchem.2022.132547
de Oliveira, S. P. A., do Nascimento, H. M. A., Sampaio, K. B., & de Souza, E. L. (2021). A review on bioactive compounds of beet (Beta vulgaris L. subsp. vulgaris) with special emphasis on their beneficial effects on gut microbiota and gastrointestinal health. Critical reviews in food science and nutrition, 61(12), 2022–2033. https://doi.org/10.1080/10408398.2020.1768510
del Amo-Mateos, E., Pérez, R., Merino, A., Lucas, S., García-Cubero, M. T., & Coca, M. (2024). Rhamnogalacturonan–I pectin and derived oligosaccharides obtained from sugar beet pulp and discarded red beetroot: Characterization and comparative study of their antioxidant and prebiotic properties. Food Hydrocolloids, 152, 109955. https://doi.org/10.1016/j.foodhyd.2024.109955
Elafify, M., Liao, X., Feng, J., Ahn, J., & Ding, T. (2024). Biofilm formation in food industries: Challenges and control strategies for food safety. Food Research International, 190, 114650. https://doi.org/10.1016/j.foodres.2024.114650
Falsafi, S. R., Bangar, S. P., Chaudhary, V., Hosseini, E., Mokhtari, Z., Karaca, A. C., Samota, M. K., Goswami, D., Krishnan, V., Askari, G., & Rostamabadi, H. (2022). Recent advances in oral delivery of bioactive molecules: Focus on prebiotic carbohydrates as vehicle matrices. Carbohydrate polymers, 298, 120074.
Flores-Mancha, M. A., Ruíz-Gutiérrez, M. G., Sánchez-Vega, R., Santellano-Estrada, E., & Chávez-Martínez, A. (2020). Characterization of Beet Root Extract (Beta vulgaris) Encapsulated with Maltodextrin and Inulin. Molecules (Basel, Switzerland), 25(23), 5498. https://doi.org/10.3390/molecules25235498
Georgiev, V. G., Weber, J., Kneschke, E. M., Denev, P. N., Bley, T., & Pavlov, A. I. (2010). Antioxidant activity and phenolic content of betalain extracts from intact plants and hairy root cultures of the red beetroot Beta vulgaris cv. Detroit dark red. Plant foods for human nutrition, 65(2), 105–111. https://doi.org/10.1007/s11130-010-0156-6
Hlikemichnyi indeks ta hlikemichne navantazhennia ovochiv. Povna tablytsia GI &
Kale, R. R., Sawate, A. R., Kshirsagar, R., Patil, B., & Mane, R. J. (2018). Studies on evaluation of physical and chemical composition of beetroot (Beta vulgaris L.). International Journal of Chemical Studies, 6, 2977–2979.
Kamel, S. M., Alqurashi, R. M., Alshebly, A. M., Saleh, F. A., Alnemr, T. M., Ali, H. F., Ali, S. A., Asiri, S. A., Ibrahim, M. E., Alqahtani, N. K., & Fernandes, F. A. N. (2024). Effect of drying method and coating with gum Arabic on physicochemical properties, betalain content, LDL oxidation, and storage stability of dried red beetroot slices. Journal of Food Processing and Preservation, Article 8471292. https://doi.org/10.1155/2024/8471292
Karpyk, H., Kukhtyn, M., Selskyi, V., Nazarko, I., Pokotylo, O., & Haidamaka, M. (2021). Research of technological properties of bread made with the addition of beet kvass. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Food Technologies, 23(96), 3–7. https://doi.org/10.32718/nvlvet-f9601
Ko, G. P., Jo, H., Kim, J., Kim, J. S., Boo, K. H., & Kim, C. S. (2024). Enterotype-Specific effects of red beetroot (Beta vulgaris L.) powder and betanin on human gut microbiota: A preliminary study based on in vitro fecal fermentation model. Life, 14(11), 1391. https://doi.org/10.3390/life14111391
Kukhtyn, M., Vichko, O., Horyuk, Y., Shved, O., & Novikov, V. (2018). Some probiotic characteristics of a fermented milk product based on microbiota of “Tibetan kefir grains” cultivated in Ukrainian household. Journal of Food Science and Technology, 55(1), 252–257. https://doi.org/10.1007/s13197-017-2931-y
Martinez, R. M., Melo, C. P. B., Pinto, I. C., Mendes-Pierotti, S., Vignoli, J. A., Verri, W. A., & Casagrande, R. (2024). Betalains: A Narrative Review on Pharmacological Mechanisms Supporting the Nutraceutical Potential Towards Health Benefits. Foods (Basel, Switzerland), 13(23), 3909. https://doi.org/10.3390/foods13233909
Milton-Laskibar, I., Martínez, J. A., & Portillo, M. P. (2021). Current Knowledge on Beetroot Bioactive Compounds: Role of Nitrate and Betalains in Health and Disease. Foods (Basel, Switzerland), 10 (6), 1314. https://doi.org/10.3390/foods10061314
Ohorodnyk, N., Svarchevska, O., Shved, O., & Kupka, T. (2023). Main aspects of the manufacturer of organic products in Ukraine. Biotechnologia Acta, 16(1), 40–50. https://doi.org/10.15407/biotech16.01.040
Olalekan, A. A., Durojaiye, O. K., & Owie, U. C. (2024). The potential role of beetroot juice in hyperglycemia management: A review of mechanisms and clinical outcomes. African Journal of Agriculture and Food Science, 7(4). https://doi.org/10.52589/ajafs-ekdavahq
Oyewole, O. A., Amole, O. F., Majin, E. N., & Maddela, N. R. (2026). Antimicrobial nanomaterials in food packaging and preservation. RSC Advances, 16(15), 13728–13748. https://doi.org/10.1039/d5ra09037g
Pochynok, A., Kisterska, L., Lohinova, O., Soloduha, A., & Pokotylo, O. (2023). Modification of synthetic knitted fabric with nanoparticles to provide antibacterial/antiviral properties to the surface. Scientific Bulletin of Uzhhorod University. Series Biology, 55, 78–89.
Postružnik, V., Stajčić, S., Borjan, D., Ćetković, G., Knez, Ž., Knez Marevci, M., & Vulić, J. (2024). Impact of storage conditions on stability of bioactive compounds and bioactivity of beetroot extract and encapsulates. Processes, 12(7), 1345. https://doi.org/10.3390/pr12071345
Prieto-Santiago, V., Cavia, M. M., Alonso-Torre, S. R., & Carrillo, C. (2020). Relationship between color and betalain content in different thermally treated beetroot products. Journal of food science and technology, 57(9), 3305–3313. https://doi.org/10.1007/s13197-020-04363-z
Punia Bangar, S., Sharma, N., Sanwal, N., Lorenzo, J. M., & Sahu, J. K. (2022). Bioactive potential of beetroot (Beta vulgaris). Food research international (Ottawa, Ont.), 158, 111556. https://doi.org/10.1016/j.foodres.2022.111556
Rudenko, A. V., Kisterska, L. D., Lohinova, O. B., Rybalko, S. L., Soloduha, H. B., & Pochynok, A. M. (2025). Providing antibacterial/antiviral properties to medical materials by modification with silver and copper nanoparticles. Chemistry, Physics and Technology of Surface, 16(1), 63–74. https://doi.org/10.15407/hftp16.01.063
Sadowska-Bartosz, I., & Bartosz, G. (2021). Biological Properties and Applications of Betalains. Molecules (Basel, Switzerland), 26(9), 2520. https://doi.org/10.3390/molecules26092520
Sarbatly, R., Sariau, J., & Krishnaiah, D. (2023). Recent Developments of Membrane Technology in the Clarification and Concentration of Fruit Juices. Food engineering reviews, 10, 1–18. Advance online publication. https://doi.org/10.1007/s12393-023-09346-2
Shved, O., Vichko, O., Karpyk, H., & Hubrii, Z. (2025). Phytoadditional supplements phyto-suppliances with bioactive phyto-extracts in food biotechnology. European Science, 2(sge 39-02), 85–115. https://doi.org/10.30890/2709-2313.2025-39-02-017
Shynkaruk, O. Yu., Kukhtyn, M. D., Vichko, O. I., Shved, O. V., & Marintsova, N. H. (2018). Characterization of the “Enzymnyi” detergent according to ability of microbial biofilms disruption. Visnyk Natsionalnoho Universytetu “Lvivska Politekhnika”. Seriia: Khimiia, Tekhnolohiia Rechovyn ta Yikh Zastosuvannia, 886, 158–164.
Sistani Karampour, N., Arzi, A., Rezaie, A., Pashmforosh, M., & Rad, H. (2019). Gastroprotective effects of betanin against ethanol-induced gastric ulcer in rats. Jundishapur Journal of Natural Pharmaceutical Products, 14(3), e14473. https://doi.org/10.5812/jjnpp.14473
Slavov, A., Karagyozov, V., Denev, P., Kratchanova, M., & Kratchanov, C. (2013). Antioxidant activity of red beet juices obtained after microwave and thermal pretreatments. Czech Journal of Food Sciences, 31(2), 139–147. https://doi.org/10.17221/61/2012-CJFS
Soja, J., & Nowak, D. (2026). Fruit Juice Processing Technologies and Their Impact on the Content of Bioactive Compounds-A Review of Current Approaches. Food science & nutrition, 14(7), e72073. https://doi.org/10.1002/fsn3.72073
Soutelino, M. E. M., da Silva, D. B., Rocha, R. da S., de Oliveira, B. C. R., Esmerino, E. A., da Cruz, A. G., Mársico, E. T., & Silva, A. C. de O. (2023). Yogurt added with beetroot extract: Physicochemical parameters, biological activities and sensory evaluation by check-all-that-apply method. International Journal of Food Science & Technology, 58(6), 3303–3309. https://doi.org/10.1111/ijfs.16214
Tyler, A. P., Linder, B. A., Ricart, K., Behrens, C. E., Ovalle, F., Patel, R. P., & Fisher, G. (2024). The effects of acute beetroot juice intake on glycemic and blood pressure responses when controlling for medication in individuals with type 2 diabetes: A pilot study. Nutrients, 16(16), 2636. https://doi.org/10.3390/nu16162636
Vaccaro, M. G., Innocenti, B., Cione, E., Gallelli, L., De Sarro, G., Bonilla, D. A., & Cannataro, R. (2024). Acute effects of a chewable beetroot-based supplement on cognitive performance: a double-blind randomized placebo-controlled crossover clinical trial. European journal of nutrition, 63(1), 303–321. https://doi.org/10.1007/s00394-023-03265-y
Vichko, O., Karpyk, H., Shved, O., Hubrii, Z., & Kopchak, N. (2024). Development of biotechnology of dairy beverages with bioactive phytoextracts of licorice. Chemistry, Technology and Application of Substances, 7(1), 154–164. https://doi.org/10.23939/ctas2024.01.154
Weinstock G. M. (2016). A Glimpse of Microbial Power in Preventive Medicine. JAMA pediatrics, 170(1), 11. https://doi.org/10.1001/jamapediatrics.2015.3246.
Wiczkowski, W., Romaszko, E., Szawara-Nowak, D., & Piskula, M. K. (2018). The impact of the matrix of red beet products and interindividual variability on betacyanins bioavailability in humans. Food Research International, 108, 530–538. https://doi.org/10.1016/j.foodres.2018.04.004
Willcox, M. L., Elugbaju, C., Al-Anbaki, M., Lown, M., & Graz, B. (2021). Effectiveness of Medicinal Plants for Glycaemic Control in Type 2 Diabetes: An Overview of Meta-Analyses of Clinical Trials. Frontiers in pharmacology, 12, 777561. https://doi.org/10.3389/fphar.2021.777561
Xianting, Li ,Yingxuan, Feng , Youyang, Ye. Min Guo, Wenwei, Lu, Jianxin, Zhao , Yue, Xiao. (2025). Wei Chen Exploring the prebiotic potential of sugar beet fiber through in vitro fecal fermentation: modulation of gut microbiota and associated metabolic signature. Food Bioscience, 73. https://doi.org/10.1016/j.fbio.2025.107768
Yong, Y. Y., Ong, M. W. K., Dykes, G., & Choo, W. S. (2021). Betacyanin-inhibited biofilm formation of co-culture of Staphylococcus aureus and Pseudomonas aeruginosa on different polymer surfaces. FEMS microbiology letters, 368(1), 214. https://doi.org/10.1093/femsle/fnaa214
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