TECHNOLOGICAL FEATURES OF THE USE OF BACTERIAL PREPARATIONS IN THE PRODUCTION OF RAW-DRIED BEEF MEAT LAYERS
DOI:
https://doi.org/10.31548/humanhealth.4.2024.28Keywords:
raw meat, meat salting, microbiological purity, plasticity, mass fraction of moisture, organoleptic quality indicatorsAbstract
It is relevant to use the combined technology of beef brine for raw products with the use of bacterial preparations, which makes it possible to reduce the content of sodium nitrite and contributes to the production of food products that are safe for consumption.
The purpose of the study is to determine the effectiveness of using a bacterial preparation that contains bacteria strains of Pediococcus acidilactici and Staphylococcus carnosus in producing raw meat flakes.
It was established that at the 8th hour of salting the beef, the pH value in the experimental sample was 5.62, which is 0.5 less than the control. At the 16th hour of salting, the pH value in the control sample decreased by 0.1; in the experimental sample, this value was 5.59. After 24 hours of salt, the pH value in the experimental sample was 5.56, which is 0.9 less than the control.
Bacteria of the group of coliforms, Salmonella, Sulfite-reducing clostridia, Listeria monocytogenes and Staphylococcus aureus were not detected in salted beef using the bacterial preparation after 72 hours of exposure, which indicates the microbiological purity of the meat raw material.
It was established that at the 8th hour of salting with a bacterial preparation, the experimental sample of beef was characterized by a lower (by 1.4%) moisture content compared to the control. After 24 hours of curing, the experimental beef sample was characterized by a moisture content of 68.8 %, which is 1.7 % less than the control. At the 16th hour of salting, the experimental sample of beef was characterized by increased plasticity by 0.2 cm2/g compared to the control. At the 24th hour of salting, the experimental sample of beef had a plasticity index increased by 0.3 cm2/g compared to the control.
Raw dried beef flakes produced using a bacterial preparation for 60 days of storage were characterized by a peroxide value of 0.85 mg-eq O2/kg, lower by 0.19 mg-eq O2/kg compared to the control. Raw dried beef flakes for 180 days of storage were characterized by a lower peroxide value (0.22 mg-eq O2/kg) than the control.
Using a bacterial preparation during the salting of beef made it possible to increase the organoleptic quality indicators of raw meat flakes.
References
Barriere, C., Leroy-Sertin, S., Talon, R. (2001). Characterization of catalase and super- oxidedismutase in Staphylococcus carnosus 833 strain. J. Appl. Microbiol, 91, 514–519. https://doi.org/10.1046/j.1365-2672.2001.01411.x
Bal-Prylypko, L. V., & Leonova, B. I. (2014). Biotechnologies of meat products production. Current state of biotechnologia acta, 7, 114–119. https://doi.org/10.15407/biotech7.05.114
Bal-Prilipko, L. V., Patyka, N. V., Leonova, B. I., Starkova, E. R., & Brona, A. I. (2016). Trends, achievements and prospects of biotechnology in the food industry. Mikrobiolohichnyi Zhurnal, 78(3), 99–111. https://doi.org/10.15407/microbiolj78.03.099
Barriere, C., Leroy-Setrin, S., & Talon, R. (2001a). Characterization of catalase and superoxide dismutase in Staphylococcus carnosus 833 strain. Journal of Applied Microbiology, 91(3), 514–519. https://doi.org/10.1046/j.1365-2672.2001.01411.x
Bortsyukh, V., & Shugai, M. (2017). Antimicrobial metabolites of lactic acid bacteria: mechanism of action and practical use. Food Resources, 5(09), 136–143. https://iprjournal.kyiv.ua/index.php/pr/article/view/202
Corbiere, M-B. S, Leroy, S., & Talon, R. (2007) Monitoring of staphylococcal starters in two French processing plants manufacturing dry fermented sausages. J Appl Microbiol, 102, 238–244. doi: 10.1111/j.1365-2672.2006.0304.
DSTU 4570 (2006). Vegetable fats and oils. The method of determining the peroxide number. Kyiv: State consumer standard of Ukraine.
DSTU GOST 30726 (2002). Food products. Methods of detection and determination of the number of bacteria of the species Escherichia coli (GOST 30726-2001, IDT). Kyiv: State consumer standard of Ukraine.
DSTU ISO 1442 (2005). Meat and meat products. Method for determining moisture content (control method) (ISO 1442:1997, IDT). With correction. Kyiv: State consumer standard of Ukraine.
DSTU ISO 2917 (2001). Meat and meat products. Determination of pH (control method) (ISO 2917:1974, IDT). Kyiv: State consumer standard of Ukraine.
DSTU EN ISO 6579-1 (2022). Microbiology of the food chain. A horizontal method for the detection, enumeration and serotyping of Salmonella. Part 1. Detection of Salmonella spp (EN ISO 6579-1:2017, IDT; ISO 6579-1:2017, IDT). Kyiv: State consumer standard of Ukraine.
DSTU EN ISO 11290-1 (2022). Microbiology of the food chain. Horizontal method of detection and counting of Listeria monocytogenes and Listeria spp. Part 1. Detection method (EN ISO 11290-1:2017, IDT; ISO 11290-1:2017, IDT). Kyiv: State consumer standard of Ukraine.
DSTU ISO 15213 (2014). Microbiology of food products and animal feed. Horizontal method for counting the number of sulfite-reducing bacteria growing under anaerobic conditions (ISO 15213:2003, IDT). Kyiv: State consumer standard of Ukraine.
Gálvez, A., López, R. L., Abriouel, H., Valdivia, E., & Omar, N. B. (2008). Application of bacteri-ocins in the control of foodborne pathogenic and spoilage bacteria. Critical Reviews in Bio-technology, 28(2), 125–152. https://doi.org/10.1080/07388550802107202
Grau, R. (1960). Fleisch und Fleischwaren. 1.ed., Berlin Verlag A.W. Hayn´s Erben.
Kolomiets, R., Strashinskyi, I., Pasichnyi, V., Dubrovatskyi, I.V., Strelchenko, L., Taradai, R., Hrytsai, M. (2015). Development Of Protein Compositions And Their Use In Canned Meat Technology. Scientific Bulletin of the LNU of Veterinary Medicine and Biotechnology. Series: Food Technology, 17(1). 37–40.
Laranjo, M., Potes, M. E., & Elias, M. (2019). Role of starter cultures on the safety of fermented meat products. Front. Microb, 10, 853. doi: 10.3389/fmicb.2019.00853
Löfblom, J., Rosenstein, R., Nguyen, R. M., Ståhl, S., & Friedrich Götz, F. (2017) Staphylococcus carnosus: from starter culture to protein engineering platform. Appl Microbiol. Biotechnol, 23, 8293-8307. doi: 10.1007/s00253-017-8528-6
Marco, A., Navarro, J. L., & Flores, M. (2006). The influence of nitrite and nitrate on microbial, chemical and sensory parameters of slow dry fermented sausage. Meat Science, 73(4), 660–673. https://doi.org/10.1016/j.meatsci.2006.03.011
Raccach, M. (2014). Pediococcus. Encyclopedia of Food Microbiology, 1–5. doi:10.1016/b978-0-12-384730-0.00247-0
Shinkaruk, M., & Baluk, O. (2021). Prospective Starter Cultures For Craft Sausage Products. Taurian Scientific Bulletin. Series: Technical Sciences, 5, 38–48.
Song, A. A., In, L. L. A., Lim, S. H. E., & Rahim, R. A. (2017). Erratum to: A review on Lactococcus lactis: From food to factory. Microbial Cell Factories, 16(1). https://doi.org/10.1186/s12934-017-0754-1
Szymański, P., Łaszkiewicz, B., Kern-Jędrychowska, A., Siekierko, U., & Kołożyn-Krajewska, D. (2021). The use of the mixed bacteria limosilactobacillus fermentum and staphylococcus carnosus in the meat curing process with a reduced amount of sodium nitrite. Applied Sciences, 11(3), 904. https://doi.org/10.3390/app11030904
Yoon, J.-W., & Kang, S.-S. (2020). In vitro antibiofilm and anti-inflammatory properties of bacteriocins produced by pediococcus acidilactici against enterococcus faecalis. Foodborne Pathogens and Disease, 17(12), 764–771. https://doi.org/10.1089/fpd.2020.2804
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