INNOVATIVE APPROACHES TO THE STAINING OF CYTOLOGICAL AND HISTOLOGICAL PREPARATIONS OF BEEF AND MEAT PRODUCTS

Abstract

The article is devoted to the development of new methods for staining cytological and histological materials for microscopic studies. Despite the variety of methods and techniques for staining histological preparations, they have a number of disadvantages associated with the quality and duration of the process. In medicine and the food industry, there is a need to quickly obtain the results of microscopic analysis, since this allows you to quickly detect changes in the patient's condition or violations of the technology of product production. Therefore, the current issue is the search for new methods and methods of staining cytological and histological samples for microscopic analysis. The purpose of the work is to develop new methodological approaches to accelerated staining of histological preparations for their further study. The work proposes the application of various types of dyes to cytological and histological material by the printing method. Peripheral blood smears, histological preparations of surgical material and preparations of meat products were used as materials for testing. All preparations were divided into two subgroups, which were separately stained by the bulk method and the printing method. For each stage of staining, the appropriate concentration of working dye solutions was selected and the time of applying the seal was set. Cytological and histological material was fixed according to the recommendations of standard methods. Before staining, histological preparations were subjected to well-known stages of deparaffinization and hydration. Analysis of stained cytological and histological material was carried out using the Vision artificial intelligence system with a digital preparation scanning module. It has been proven that the use of the seal method in staining cytological and histological material is an innovative solution in histological practice, refuting the indisputable advantage of the bulk staining method. It has been shown that the use of seal staining is universal for both cytological and histological material. The use of scanning and the creation of digital preparations revealed significant advantages in their quality compared to traditional methods. The practical value of the seal staining method lies in a significant reduction in staff time, a decrease in the amount of reagents and related materials used.