AMINO ACID COMPOSITION OF COOKED SAUSAGE PRODUCTS ENRICHED WITH CHLORELLA
DOI:
https://doi.org/10.31548/humanhealth.1.2025.126Keywords:
cooked sausage, seaweed, protein, essential amino acids, biological valueAbstract
Evaluation of the amino acid composition of cooked sausage products is an important component for determining their nutritional value and nutritional properties of the finished product. Promising in the technology of cooked sausages is the use of chlorella, which prevails in the amino acid composition of certain types of traditionally used seaweed. The aim of the article is to study the amino acid composition of sausage products with the addition of chlorella. The mass fraction of essential amino acids in the control and experimental samples was determined by chromatographic method. The amino acid ratio, its coefficient of variation, and biological value were determined by the calculation method. According to the results of determining the content of essential amino acids, it was found that experimental sample 1 has a higher amount of them compared to the control, namely histidine - by 7,37 %, isoleucine - by 25.04 %, lysine - by 19.75 %, leucine - by 15.63 %, threonine - by 10.06 %, valine - by 7.26 %, methionine - by 7.00 %, tryptophan - by 3.60 %, phenylalanine - by 2.07 %. It was found that the developed samples of sausage products with the addition of chlorella are a source of leucine, lysine, and isoleucine. The calculation of the amino acid ratio in the experimental samples showed that all essential amino acids have a value of 100 % and above, the limited amino acid is threonine, whose ratio is 102 % for sample 1 and 101 % for sample 2, and the highest ratio belongs to histidine - 175 % for the first sample and 165 % for the second. It was found that the coefficient of amino acid scoring in experimental samples 1 and 2 is lower by 2.88 % and 1.34 % compared to the control, and the indicators of biological value are respectively higher by 2.88 % and 1.34 % compared to the control. Thus, the introduction of chlorella into the formulation allows to improve the amino acid composition of the finished sausage product due to a balanced set of essential amino acids
References
Asioli, D., Aschemann-Witzel, J., Caputo, V., Vecchio, R., Annunziata, A., Næs, T., & Varela, P. (2017). Making sense of the “clean label” trends: A review of consumer food choice behavior and discussion of industry implications. Food Research International, 99, 58-71. http://dx.doi.org/10.1016/j.foodres.2017.07.022
Boye, J., Wijesinha-Bettoni, R., & Burlingame, B. (2012). Protein quality evaluation twenty years after the introduction of the protein digestibility corrected amino acid score method. British Journal of Nutrition, 108(S2), S183-S211. http://dx.doi.org/10.1017/s0007114512002309
Cofrades, S., López‐López, I., & Jiménez‐Colmenero, F. (2011). Applications of seaweed in meat‐based functional foods. Handbook of marine macroalgae: Biotechnology and applied phycology, 491-499. http://dx.doi.org/10.1002/9781119977087.ch32
Dawczynski, C., Schubert, R., & Jahreis, G. (2007). Amino acids, fatty acids, and dietary fibre in edible seaweed products. Food chemistry, 103(3), 891-899. https://doi.org/10.1016/j.foodchem.2006.09.041
De Gavelle, E., Huneau, J. F., Bianchi, C. M., Verger, E. O., & Mariotti, F. (2017). Protein adequacy is primarily a matter of protein quantity, not quality: modeling an increase in plant: animal protein ratio in French adults. Nutrients, 9(12), 1333. https://doi.org/10.3390/nu9121333
DSTU 4436:2005. Boiled sausages, sausages, anchovies, meat loaves.
DSTU ISO 13903 (2009). Fodder for animals. Method for determining the content of amino acids (ISO 13903:2005, IDT).
Fleurence, J., Morançais, M., & Dumay, J. (2018). Seaweed proteins. In Proteins in food processing (pp. 245-262). Woodhead Publishing. http://dx.doi.org/10.1016/b978-0-08-100722-8.00010-3
Fujiwara-Arasaki, T., Mino, N., & Kuroda, M. (1984). The protein value in human nutrition of edible marine algae in Japan. In Eleventh International Seaweed Symposium: Proceedings of the Eleventh International Seaweed Symposium, held in Qingdao, People’s Republic of China, June 19–25, 1983 (pp. 513-516). Springer Netherlands.
Gilani, G. S., Xiao, C. W., & Cockell, K. A. (2012). Impact of antinutritional factors in food proteins on the digestibility of protein and the bioavailability of amino acids and on protein quality. British Journal of Nutrition, 108(S2), S315-S332. https://doi.org/10.1017/S0007114512002371
Han, S. W., Chee, K. M., & Cho, S. J. (2015). Nutritional quality of rice bran protein in comparison to animal and vegetable protein. Food chemistry, 172, 766-769. https://doi.org/10.1016/j.foodchem.2014.09.127
Kakinuma, M., Park, C. S., & Amano, H. (2001). Distribution of free L-cysteine and glutathione in seaweeds. Fisheries science, 67(1), 194-196. https://doi.org/10.1046/j.1444-2906.2001.00223.x
Kolb, N., Vallorani, L., & Stocchi, V. (1999). Chemical composition and evaluation of protein quality by amino acid score method of edible brown marine algae Arame (Eisenia bicyclis) and Hijiki (Hijikia fusiforme). Acta Alimentaria, 28(3), 213-222. http://dx.doi.org/10.1556/aalim.28.1999.3.1
Kulakova, L., & Slyva, Y. (2024). Development of formulations of cooked sausage products enriched with chlorella. Human and nation's Health, 3, 84-93. https://doi.org/10.31548/humanhealth.3.2024.84
MacArtain, P., Gill, C. I., Brooks, M., Campbell, R., & Rowland, I. R. (2007). Nutritional value of edible seaweeds. Nutrition reviews, 65(12), 535-543. https://doi.org/10.1111/j.1753-4887.2007.tb00278.x
Marinangeli, C. P., Foisy, S., Shoveller, A. K., Porter, C., Musa-Veloso, K., Sievenpiper, J. L., & Jenkins, D. J. (2017). An appetite for modernizing the regulatory framework for protein content claims in Canada. Nutrients, 9(9), 921. http://dx.doi.org/10.3390/nu9090921
Mišurcová, L., Buňka, F., Ambrožová, J. V., Machů, L., Samek, D., & Kráčmar, S. (2014). Amino acid composition of algal products and its contribution to RDI. Food chemistry, 151, 120-125. http://dx.doi.org/10.1016/j.foodchem.2013.11.040
Nenova, N., & Drumeva, M. (2012). Investigation on protein content and amino acid composition in the kernels of some sunflower lines. Helia, 35(56), 41-46. http://dx.doi.org/10.2298/hel1256041n
Nosworthy, M. G., Neufeld, J., Frohlich, P., Young, G., Malcolmson, L., & House, J. D. (2017). Determination of the protein quality of cooked Canadian pulses. Food science & nutrition, 5(4), 896-903. https://doi.org/10.1002/fsn3.473.
Sá, A. G. A., Moreno, Y. M. F., & Carciofi, B. A. M. (2020). Plant proteins as high-quality nutritional source for human diet. Trends in Food Science & Technology, 97, 170-184. http://dx.doi.org/10.26782/jmcms.spl.10/2020.06.00048
Smith, C. R., Shekleton, M. C., Wolff, I. A., & Jones, Q. (1959). Seed protein sources—amino acid composition and total protein content of various plant seeds. Economic Botany, 13(2), 132-150. http://dx.doi.org/10.1007/bf02859245
Vendemiatti, A., Rodrigues Ferreira, R., Humberto Gomes, L., Oliveira Medici, L., & Antunes Azevedo, R. (2008). Nutritional quality of sorghum seeds: Storage proteins and amino acids. Food Biotechnology, 22(4), 377-397. https://doi.org/10.1080/08905430802463487
Who, J. (2007). Protein and amino acid requirements in human nutrition. World Health Organization technical report series, (935), 1.
Wolfe, R. R., Rutherfurd, S. M., Kim, I. Y., & Moughan, P. J. (2016). Protein quality as determined by the Digestible Indispensable Amino Acid Score: evaluation of factors underlying the calculation. Nutrition reviews, 74(9), 584–599. https://doi.org/10.1093/nutrit/nuw022
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